02 Jan New NIH grant awarded
We are excited to announce FilamenTech’s participation in a new scientific project called “Analysis Tools for Fiber Diffraction of Muscle” (R01GM144555), supported by National Institute of General Medical Sciences of the National Institutes of Health. Principal investigator is Thomas C. Irving from Illinois Institute of Technology, Chicago, IL, USA.
The aim of the project is to build robust, user-friendly tools for data reduction and computational tools for modeling diffraction patterns, broadly applicable to all muscle systems without a specific disease focus, in order to interpret X-ray diffraction experimental data. This would lead to increase of the efficiency and reproducibility of data from muscle fiber diffraction experiments on muscle and provide a potent hypothesis generation and testing tool that can greatly increase the value of past, present, and future X-ray diffraction experiments on muscle.
FilamenTech will be in charge of developing a communication protocol from the computational simulation package MUSICO, that provides all molecular positions at a given time point in a simulation, to the MUSICO-X package designed to predict X-ray diffraction patterns. We will extend thin filament simulation procedure for predicting X-ray patterns, currently implemented in MUSICO-X, to develop new procedures and software modules incorporated into the MUSICO-X package that will enable simulation of the diffraction patterns from:
• Regulated thin filaments with troponin and tropomyosin, and thick filaments activation with kinetic changes in “parked (myosin) state”.
• The effect of the auxiliary proteins nebulin, associated with thin filament (only in skeletal muscle), and MyBP-C and titin, associated the thick filament (in both skeletal and cardiac muscle).